Products for Research Use Only

CHRNA7 Antibody

CAT: 0013-GTR18225314Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225314Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CHRNA7 Antibody
Product Name Alternative
CHRNA7, AChR-alpha7, Acra 7, CHRNA7-2, NAChR alpha 7, NACHRA7, a7nAchR
Reactivity
Bovine, Canine, Equine, Gallus, Human, Monkey, Porcine
Immunogen
Peptide with sequence KRPGEDKVRPACQHKQ, from the internal region of the protein sequence according to NP_000737.1.
Target
CHRNA7
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (3 - 6 μg/ml), Peptide-ELISA (1:128000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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