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EGFR Antibody

CAT: 0013-GTR18225141Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225141Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EGFR Antibody
Product Name Alternative
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Reactivity
Gallus, Human, Mouse, Rat
Immunogen
A synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region
Target
EGFR
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
ICC, IHC, IHC-P (5 μg/ml), IP (10 - 12 μg/ml), WB
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.
Tested Applications
ICC, IHC, IHC-P, IP, WB
Host or Source
Sheep
Preservative
PBS

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