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PCNA Antibody

CAT: 0013-GTR18224470Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18224470Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PCNA Antibody
Product Name Alternative
PCNA, Cyclin
Reactivity
Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat
Immunogen
Peptide with sequence C-NGNIKLSQTSNVD, from the internal region of the protein sequence according to NP_002583.1.
Target
PCNA
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
ELISA, IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line HeLa (calculated MW of 28.8kD according to NP_002583.1) . Approx. 33kD band was observed in lysates of cell line NIH3T3 and of Mouse and Rat Testis. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186 (5) :645-54.) . Recommended concentration: 0.01-0.03 ug/ml. Enzyme immunoassay: This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with This.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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