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MSLN/Mesothelin Antibody

CAT: 0013-GTR18223848Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18223848Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MSLN/Mesothelin Antibody
Product Name Alternative
MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1
Reactivity
Human
Immunogen
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Target
MSLN / Mesothelin
Clonality
Monoclonal
Clone
MN-1
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Concentration
1 mg/ml
Dilution
ELISA (1:10000 - 1:50000), IHC, IHC-P (15 μg/ml), WB (1:1000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Isotype
IgG2a

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