Mouse HC II (Heparin Cofactor II) CLIA Kit

• Abbreviation:

  HCII

• Cross Reactivity:

  This kit recognizes Mouse HCII in samples. No significant cross-reactivity or interference between Mouse HCII and analogues was observed

• Type:

  Sandwich-ELISA

• Applications:

  CLIA

• Assay Principle:

  This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HCII. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HCII and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HCII, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HCII. You can calculate the concentration of Mouse HCII in the samples by comparing the RLU value of the samples to the standard curve.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  31.25-2000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  18.75 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C/-20°C,6 months

• Species:

  Mouse

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Mouse HC II concentrations