EasyStep Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit

EasyStep Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2, and the Human EGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR2. After TMB substrate solution is added, only those wells that contain Human EGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
• Field of Research: Signal transduction; Tumor immunity; Reproductive science; Hepatology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 1.5h
• Standard: 5000 pg/mL
• Sample Type: Serum, plasma
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 16.9 pg/mL