Recombinant Halobacterium salinarum Cobalamin import system permease protein BtuC (btuC)

Recombinant Halobacterium salinarum Cobalamin import system permease protein BtuC (btuC)

• CAS Number: 9000-83-3
• Gene Name: btuC
• UniProt: B0R5G3
• Expression Region: 1-369aa
• Organism: Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
• Target Sequence: MREASARAVAWSAAAGVLLVAVLLVSATIGPEPITLRTVAMAALTELAVPVGASVTMHTHAVPVVSGGLPWPALTIAYAAPLQFGVPETAQVIVGTIRLPRIVLGATVGASLAISGAVLQGFFRNPMADPSIVGVSSGAAVGAVAAITLPSVVVIGVQPAAFAGALIAAFTVYAIATKNGHTPTATLLLSGVAVQTLLGAVTSFLVVNSGREIRPAMYWLMGTLHGSRWHDVEAALPVVVVGSAVLLAYAREMNVLLAGEEDAHTLGVDVDRTKRLLLAVASVVTAAAVSFAGAIGFVGLIVPHAVRLVVGPDHRVLLPVSALTGGAFLVAADTVARATATEPPVGIITALIGAPFFLYLLRDREVRAL
• Tag: N-terminal 10xHis-tagged
• Source: in vitro E.coli expression system
• Field of Research: Others
• Assay Type: CF Transmembrane Protein & Developed Protein
• Relevance: Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably involved in the translocation of the substrate across the membrane
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 40.3 kDa
• References & Citations: "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346 (2008)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.