Human FGF basic Protein, premium grade

CAT: 0716-BFF-H4117-1mgSize: 1 mgDry Ice: NoHazardous: No
CAT#:0716-BFF-H4117-1mgSize:1 mg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
Description
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
Specifications
This protein carries no "tag". The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .
Accession Number
P09038-4
Expression Region
Pro 143 - Ser 288
Host
E. coli
Target
FGF basic
Conjugation
Unconjugated
Tag
Native
Source
Human
Stability
-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.01 EU per μg
Purity
95%
Bioactivity
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag (Cat. No. GP3-H5258) with a linear range of 0.3-5 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
16.5 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
250ug*4
Host or Source
E. coli
Species
Human
Protein ID
P09038-4
Preservative
Trehalose

Related Products

CatalogName
BFF-H5115-1mgHuman FGF basic (154aa) Protein, premium grade
BFF-H5115-20ugHuman FGF basic (154aa) Protein, premium grade
BFF-H5115-50ugHuman FGF basic (154aa) Protein, premium grade
BFF-H5113-50ugHuman FGF basic Superior Stable Mutant Protein, premium grade
BFF-H5113-1mgHuman FGF basic Superior Stable Mutant Protein, premium grade
BFF-H5113-20ugHuman FGF basic Superior Stable Mutant Protein, premium grade
FGB-H5115-1mgHuman FGF-8b Protein, premium grade
FGB-H5115-100ugHuman FGF-8b Protein, premium grade
BFF-H4118-20ugHuman FGF basic Protein, Research Grade
BFF-H4118-50ugHuman FGF basic Protein, Research Grade

Alternative Products

CatalogName
F228-1EAFORCEPS, DRESSING, 6 in, PREMIUM GRADE
S988-1EASCISSORS, STRAIGHT, 6 in, PREMIUM GRADE
F261-1EAFORCEPS, DRESSING, 8 in, PREMIUM GRADE
30611028-1Fetal Bovine Serum, Premium Grade
F272-1EAFORCEPS, DRESSING, 10 in, PREMIUM GRADE
F315-1EAFORCEPS, BAYONET, 8.25 in, PREMIUM GRADE
F208-1EAFORCEPS, DRESSING, 4.75 in, PREMIUM GRADE
F3158-1EAFORCEPS, DRESSING, 9.5 in, PREMIUM GRADE
P06-27020Human Serum Albumin (HSA), 20 % Solution, Premium Grade
NB-17455-03Collagenase NB 1 Premium Grade from Clostridium histolyticum

Popular Products