Human Normal Tissue Blot V

CAT: 0223-1525Size: 1 EachDry Ice: NoHazardous: No
CAT#:0223-1525Size:1 Each
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Background
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.
Type
Immunoblots
Shipping Conditions
Room Temp
Storage Conditions
Store at 4°C. The blot can be reused after stripping.
Applications Notes
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Tested Applications
WB
Species
Human

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