Human Normal Tissue Blot V

• Background:

  The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.

• Type:

  Immunoblots

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Store at 4°C. The blot can be reused after stripping.

• Applications Notes:

  For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.

• Tested Applications:

  WB

• Species:

  Human