Lymph node Membrane Lysate

• Background:

  Human lymph node tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human lymph node tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚ C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated lymph node tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated lymph node tissue membrane protein is then Western analyzed by either GAPDH or β -actin antibody to confirm there is no signal or very weak signal.

• Type:

  Lysates

• Concentration:

  2 mg/mL or better

• Buffer:

  HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

• Applications Notes:

  Lymph node membrane protein lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Liquid