K562 Cytoplasmic Lysate

• Background:

  K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) Cell cytoplasmic Protein Lysate cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated K562 (Human Chronic Myelogenous Leukemia; Bone Marrow) tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

• Type:

  Lysates

• Concentration:

  2 mg/mL or better

• Buffer:

  HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

• Applications Notes:

  K562 (Chronic Myelogenous Leukemia; Bone Marrow) Cell Cytoplasmic protein lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Liquid