Brain Lysate

• Background:

  Rabbit brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚ C. The rabbit brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β -actin antibody, and the expression level is consistent with each lot.

• Type:

  Lysates

• Concentration:

  2 mg/mL or better

• Buffer:

  HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

• Applications Notes:

  Brain lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.

• Tested Applications:

  WB

• Species:

  Rabbit

• Physical Properties:

  Liquid