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Colon Tissue Lysate (Normal)

CAT: 0223-1715-17Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-1715-17Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μ g/ml of aprotinin, 5 μ g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β -mercaptoethanol.
Type
Lysates
Concentration
2 mg/mL
Buffer
Colon Tissue Lysate (Normal) is supplied in SDS sample buffer containing 5% β -mercaptoethanol.
Shipping Conditions
Blue Ice
Storage Conditions
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Tested Applications
WB
Species
Human
Physical Properties
Liquid

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