Duodenum Tumor Lysate

• Background:

  Duodenum tumor tissue lysate was prepared by homogenization in lysis buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 μ g/ml aprotinin, 5 μ g/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS) . Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

• Type:

  Lysates

• Concentration:

  2 mg/mL

• Buffer:

  Duodenum Tumor lysate is supplied in SDS sample buffer containing 50 mM DTT.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Liquid