UCHL1 Recombinant Protein

• Background:

  Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also, UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.

• NCBI Gene ID:

  7345

• Swiss Prot:

  P09936

• Accession Number:

  NP_004172

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Autophagy, Cell Cycle, Immunology, Neuroscience, Other

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  20mM Tris, 500mM Nacl,1mM DTT, pH 8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -71˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  25.7 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Choi J., et al., 2004, J. Biol. Chem. 279:13256-13264.

• Background Reference 02:

  Liu Y., et al., 2002, Cell 111:209-218.

• Background Reference 03:

  Choudhary C., et al., 2009, Science 325:834-840.

• Other Product Names:

  UCHL1, PGP9.5

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3