Onchocerca volvulus OV16 Recombinant Protein

• Background:

  The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.

• Tag:

  GST Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  46.8 kDa

• Applications Notes:

  This protein carries a GST tag at the N-terminus. The protein has a calculated MW of 46.8 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Cama, Vitaliano A., et al., 2018, Am. J. Trop. Med. Hyg., 1-8.

• Background Reference 02:

  Miley, Kristi M., 2017, Graduate Theses and Dissertations., 1-42.

• Background Reference 03:

  E Lobos., at al., 1991, Science, 251:1603-1605.

• Other Product Names:

  OV16, OV-16 antigen

• Tested Applications:

  WB

• Species:

  Onchocerca volvulus

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3