SIRP beta / CD172b Recombinant Protein

• Background:

  Signal-regulatory protein beta-1 (SIRP beta 1) also known as SIRPβ1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily. SIRPβ1 is a transmembrane protein that has three Ig-like domains in its extracellular region and a short cytoplasmic tail. Human SIRPβ1 is expressed on monocytes and granulocytes but not on lymphocytes. SIRP beta 1 contains short cytoplasmic domains that lack cytoplasmic sequence motifs capable of recruiting SHP-2 and SHP-1. In addition, they contain a single basic lysine residue within the hydrophobic transmembrane domain.

• NCBI Gene ID:

  10326

• Swiss Prot:

  Q5TFQ8

• Accession Number:

  NP_006056.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Signal Transduction

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  63.7 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Signal regulatory protein beta 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gaikwad S., et al. 2009, Am J Pathol., 175 (6), 2528-39.

• Background Reference 02:

  Dietrich J., et al. 2000, J Immunol., 164 (1), 9-12.

• Background Reference 03:

  Tomasello E., et al. 2000, Eur J Immunol., 30 (8), 2147-56.

• Other Product Names:

  CD172b antigen, CD172b, DKFZp686A05192, FLJ26614, SIRP beta 1, SIRPB1, SIRP-BETA-1,9930027N05Rik, SIRP-beta

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3