RCAN2 Isoform 2 Recombinant Protein

• Background:

  Regulator of calcineurin 2 (RCAN2), also known as ZAKI4 and DSCR1L1, is expressed as two isoforms differing at their N-terminus. The longer of the two (isoform 1) is expressed exclusively in the brain, while isoform 2 is ubiquitously expressed, with highest expression in brain, heart, and muscle (1,2) . Both isoforms bind to the catalytic subunit of calcineurin, a Ca++-dependent protein phosphatase involved in several neuronal functions, though their C-terminal region and inhibit calcineurin’s activity (3) . Unlike isoform 1 of RCAN2, the expression of the second isoform is not induced by the thyroid hormone T3 (3) . RCAN2 is a member of a family of three endogenous calcineurin regulators that are located near the minimal supernumerary fragment of chromosome 21 in individuals with Down syndrome, suggesting that they play a role in this syndrome (4) .

• NCBI Gene ID:

  53901

• Swiss Prot:

  Q9JHG2

• Accession Number:

  AAH62141

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Neuroscience

• Purity:

  ~95%

• Buffer:

  100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% gycerol

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  26 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Regulator of calcineurin 2

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Miyazaki T, Kanou Y, Murata Y, et al. Molecular cloning of a novel thyroid hormone-responsive gene, ZAKI-4, in human skin fibroblasts. J. Biol. Chem. 1996; 271:14567-71.

• Background Reference 02:

  Rothermal B, Vega RB, Yang J, et al. A protein encoded within the Down syndrome critical region is enriched in striated muscles and inhibits calcineurin signaling. J. Biol. Chem. 2000; 275:8719-25.

• Background Reference 03:

  Cao X, Kambe F, Miyazaki Y, et al. Novel human ZAKI-4 isoforms: hormonal and tissue-specific regulation and function as calcineurin inhibitors. Biochem. J. 2002; 367:459-66.

• Background Reference 04:

  Fuentes JJ, Genesca L, Kingsbury TJ, et al. DSCR1, overexpressed in Down syndrome, is an inhibitor of calcineurin-mediated signaling pathways. Hum. Mol. Genet. 2000; 9:1681-90.

• Other Product Names:

  RCAN2 Antibody: Csp2, MCIP2, ZAKI-4, Dscr1l1, Zaki4, Calcipressin-2, Calcineurin inhibitory protein ZAKI-4

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Protein ID:

  38328420

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3