TNFSF11 / RANKL / CD254 Recombinant Protein

• Background:

  Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

• NCBI Gene ID:

  8600

• Swiss Prot:

  O14788

• Accession Number:

  AAC51762.1

• Tag:

  Mouse IgG Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  47.4 kDa

• Applications Notes:

  This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  TNFsuperfamilymember11

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hanada R., et al., 2011, J. Mol. Med. 89 (7) : 647-656.

• Background Reference 02:

  Findlay DM., et al., 2011, Osteoporos Int. 22 (10) : 2597-2602.

• Background Reference 03:

  Schmiedel BJ., et al., 2013, Cancer Res. 73 (2) : 683-694.

• Background Reference 04:

  Castellano D., et al., 2011, Oncologist. 16 (2) : 136-145.

• Other Product Names:

  RANKL, CD254, TRANCE, OPGL, ODF

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3