SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein

• Background:

  Nucleocapsid protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we identified as the viral nucleocapsid (N) protein. Novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. The array of diverse functional activities accommodated in the hantaviral N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

• NCBI Gene ID:

  43740575

• Accession Number:

  QHO62115.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Infectious Disease, COVID-19

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  49.4 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Nucleocapsid phosphoprotein

• NCBI Organism:

  Severe acute respiratory syndrome coronavirus 2

• Background Reference 01:

  Reuter M, et al. Virus Genes. 2018. 54:5-16.

• Background Reference 02:

  Grunewald ME, et al. Virology. 2018. 517:62-68.

• Background Reference 03:

  Jeeva S, et al. PLoS One. 2017. 12 (9) :e0184935.

• Other Product Names:

  Nucleocapsid protein, NP, Protein N

• Tested Applications:

  WB

• Species:

  SARS-CoV-2

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3