Interleukin 1b Recombinant Protein

• Background:

  Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase) . Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE) . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

• NCBI Gene ID:

  3553

• Swiss Prot:

  P01584

• Accession Number:

  NP_000567

• Tag:

  Tag free

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Cancer, Chemokines & Cytokines, Immunology, Innate Immunity, Neuroscience, Obesity, Signal Transduction

• Purity:

  >97% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  17.5 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Interleukin 1, beta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Auron PE, Webb AC. Proc Natl Acad Sci U S A 81 (24) .

• Background Reference 02:

  March CJ, Mosley B, Larsen A. Nature 315 (6021) : 641–7.

• Background Reference 03:

  Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE. Nucleic Acids Res 14 (20) : 7897–1914.

• Background Reference 04:

  Martinon, F. and J. Tschopp, 2007, Cell Death Differ. 14:10.

• Other Product Names:

  IL1B, IL-1BETA, IL1F2, IL-1β

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3