Hemagglutinin Recombinant Protein

• Background:

  Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1) . Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, but it is in birds that all subtypes can be found (2) . These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with host cell surface proteins containing oligosaccharides with terminal sialyl residues. Its extracellular region has two domains (HA1 and HA2) ; HA1 is cleaved from the main hemagglutinin protein by the host immune system. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3) . This more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in Southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the efficiency of this type of transmission is low (4) . Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5) . This HA1 recombinant protein is recognized by several ProSci hemagglutinin antibodies.

• NCBI Gene ID:

  3654620

• Swiss Prot:

  Q692M2

• Accession Number:

  AAT76166

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Infectious Disease

• Purity:

  ~95%

• Buffer:

  3

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  39 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Hemagglutinin

• NCBI Organism:

  Influenza A virus (A/chicken/Jilin/9/2004 (H5N1) )

• Background Reference 01:

  Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.

• Background Reference 02:

  Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13.

• Background Reference 03:

  Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virol. 1998; 252:331-342.

• Background Reference 04:

  Buxton Bridges C, Katz JM, Seto WH, et al. Risk of influenza A (H5N1) infection among health care workers exposed to patients with influenza A (H5N1), Hong Kong. J. Inf. Dis. 2000; 181:344-8.

• Other Product Names:

  H5N1 Hemagglutinin HA1, H5N1 Hemagglutinin, H5N1 HA1, HA1

• Tested Applications:

  ELISA, WB

• Species:

  Influenza A virus (A/chicken/Jilin/9/2004 (H5N1) )

• Protein ID:

  50365729

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3