B7-H2 / ICOSLG Recombinant Protein

• Background:

  ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

• NCBI Gene ID:

  23308

• Swiss Prot:

  O75144

• Accession Number:

  NP_056074.1

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  53.1 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Inducible T cell costimulator ligand

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Aicher A., et al., 2000, J. Immunol. 164:4689-4696.

• Background Reference 02:

  Yoshinaga SK., et al., Int Immunol 12 (10) : 1439–47.

• Other Product Names:

  ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3