G-CSF Recombinant Protein

• Background:

  Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK) /signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) /protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC) . In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells) . The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.

• NCBI Gene ID:

  1440

• Swiss Prot:

  P09919

• Accession Number:

  NP_757373.1

• Tag:

  Tag Free

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Chemokines & Cytokines

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  18.7 kDa

• Applications Notes:

  This recombinant protein can be used for E, WB. For research use only.

• NCBI Official Name:

  Granulocyte colony-stimulating factor isoform b precursor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Schneider A, , et al., 2005, J. Clin. Invest. 115 (8) : 2083–98.

• Background Reference 02:

  Pitzer C, et al., 2008, Brain 131 (Pt 12) : 3335–47.

• Other Product Names:

  G-CSF, CSF3, C17orf33, CSF3OS, MGC45931

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3