Glypican 2 / GPC2 Recombinant Protein

• Background:

  Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

• NCBI Gene ID:

  71951

• Swiss Prot:

  Q8BKV1

• Accession Number:

  NP_766000.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  60.6 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.6 kDa. The protein migrates as 60-106 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Glypican 2 (cerebroglycan)

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Herndon ME, et al. 1999. Glycobiology. 9 (2) :143-55.

• Background Reference 02:

  Li N, et al. 2017. Proc Natl Acad Sci U S A. 114 (32) :E6623-E6631.

• Background Reference 03:

  Bosse KR, et al. 2017. Cancer Cell. 32 (3) :295-309.

• Other Product Names:

  Glypican 2, GPC2

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3