Glypican 1 / GPC1 Recombinant Protein

• Background:

  Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

• Accession Number:

  NP_002072.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  82.5 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 82.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Vermeesch JR, et al. 1995. Genomics. 25:327-9.

• Background Reference 02:

  Ronca F, et al. 2001. J Biol Chem. 276 (31) :29141-7.

• Background Reference 03:

  Taylor DR, et al. 2009. PLoS Pathog. 5 (11) :e1000666.

• Other Product Names:

  Glypican 1, GPC1

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3