E-Selectin Recombinant Protein

• Background:

  E-Selectin is also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein.[1] It is transiently expressed on vascular endothelial cells in response to IL-1beta and TNFalpha. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor) -like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto) .[2] During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-alpha by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels.[3] E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

• NCBI Gene ID:

  6401

• Swiss Prot:

  P16581

• Accession Number:

  NP_000441.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Buffer:

  50 mM Tris, 100 mM Glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  84.8 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  E-selectin precursor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Collins T, et al., 1991, J. Biol. Chem. 266 (4) : 2466–73.

• Background Reference 02:

  Graves BJ, et al., 1994, Nature 367 (6463) : 532–8.

• Background Reference 03:

  Robbins SL, et al., 1999, Philadelphia: WB Saunders. ISBN 0-7216-7335-X.

• Other Product Names:

  SELE, RP1-117P20.2, CD62E, ELAM1, ESEL, LECAM2, E-Selectin

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3