Ebolavirus Glycoprotein Recombinant Protein

• Background:

  EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L) . GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP) . GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor (s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

• NCBI Gene ID:

  279888

• Swiss Prot:

  P87666.1

• Accession Number:

  AAQ55048.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Infectious Disease

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  51.6 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Virion spike glycoprotein precursor

• NCBI Organism:

  Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

• Background Reference 01:

  Saeed M.F., et al., 2010, PLoS Pathog. 6:0-0.

• Background Reference 02:

  Marzi A., et al., 2006, J. Virol. 80:6305-6317.

• Background Reference 03:

  Chandran K., et al., 2005, Science 308:1643-1645.

• Other Product Names:

  Ebolavirus Glycoprotein

• Tested Applications:

  WB

• Species:

  Ebolavirus

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3