Annexin A5 Recombinant Protein

• Background:

  Anexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Anexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Anexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Anexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc) .

• NCBI Gene ID:

  308

• Swiss Prot:

  P08758

• Accession Number:

  AAH01429

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Cancer, Cell Cycle, Other

• Purity:

  >98% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  36.8 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Annexin A5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Adekunle I. Elegbede a, D.K. Srivastava, et al. 2006, Protein Expression and PuriWcation 50: 157–162.

• Background Reference 02:

  Bent Brachvogela, Heike Welzel, et al. 2001, Mechanisms of Development 109: 389–393.

• Other Product Names:

  ANXA5, ANX5, ENX2, PP4, Annexin V, Annexin A5

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3