Human Netrin receptor DCC / DCC Protein, Fc Tag (MALS verified)

• Background:

  Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.

• Description:

  Human Netrin receptor DCC, Fc Tag (NEC-H5254) is expressed from human 293 cells (HEK293) . It contains AA Phe 32 - Asn 1097 (Accession # P43146-1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 144.1 kDa. The protein migrates as 140-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P43146-1

• Expression Region:

  Phe 32 - Asn 1097

• Host:

  HEK293

• Target:

  Netrin receptor DCC

• Conjugation:

  Unconjugated

• Tag:

  C-Fc

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human Netrin-1, His Tag (Cat. No. NE1-H52H3) at 5 μg/mL (100 μL/well) can bind Human Netrin receptor DCC, Fc Tag (Cat. No. NEC-H5254) with a linear range of 1-10 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  144.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Species:

  Human