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CD274 Antibody (Biotin)

CAT: 0013-GTR18196241Size: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18196241Size:0.5 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse monoclonal antibody against CD274 conjugated to Biotin
Product Name Alternative
Anti-B7 homolog 1 antibody, anti-B7-H1 antibody, anti-CD274 antibody, anti-PD-L1 antibody, anti-PDCD1 ligand 1 antibody, anti-PDCD1L1 antibody, anti-PDCD1LG1 antibody, anti-Programmed cell death 1 ligand 1 antibody
UniProt
Q9NZQ7
Reactivity
Human, Mouse
Immunogen
Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
Target
CD274
Clonality
Monoclonal
Clone
PDL1/2746
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
Protein A
Concentration
Purified Ab conjugated to Biotin
Dilution
Flow Cytometry (1-2ug/million cells) ; , Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
37-50kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Jurkat, RAW, HEK293 or HepG2 cells. Human lung SqCC, cervical or breast carcinoma.
Tested Applications
ELISA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Isotype
IgG2b
Entrez
29126

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