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NT5E Antibody (Biotin)

CAT: 0013-GTR18195067Size: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18195067Size:0.5 mL
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Description
Mouse monoclonal antibody against NT5E conjugated to Biotin
Product Name Alternative
Anti-5&apos antibody, anti-NT antibody, anti-5&apos antibody, anti-nucleotidase (CD73) antibody, anti-5&apos antibody, anti-nucleotidase precursor antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto (CD73) antibody, anti-E5NT antibody, anti-Ecto 5&apos antibody, anti-nucleotidase antibody, anti-Ecto-5&apos antibody, anti--nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT5 antibody, anti-NT5E antibody, anti-NTE antibody, anti-Purine 5 Prime Nucleotidase antibody
UniProt
P21589
Reactivity
Human
Immunogen
Recombinant full-length human NT5E protein
Target
NT5E
Clonality
Monoclonal
Clone
NT5E/2646
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
Protein G
Concentration
Purified Ab conjugated to Biotin
Dilution
ELISA (For coating, order antibody without BSA) ; Flow Cytometry (2-4ug/million cells in 0.1ml) ; , Immunofluorescence (2-4ug/ml) ; Western Blot (2-4ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Molecular Weight
71kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: A431, U87MG or PC-3 cells. Human tonsil, prostate or lung carcinoma.
Tested Applications
ELISA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Isotype
IgG1
Entrez
4907

UniProtKB · P21589

5'-nucleotidase

5NTD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P21589
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NT5E
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
5'-NT
EC number
3.1.3.35, 3.1.3.5, 3.1.3.89, 3.1.3.91, 3.1.3.99
Processing
Precursor
Secondary accessions
B3KQI8, O75520, Q5W116
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

Disease

Disease variant

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Molecular function

Hydrolase

Ligand

Metal-bindingNucleotide-bindingZinc

Domain

Signal

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