Cattle IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit

Cattle IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: BP4; HT29-IGFBP; IBP4
• UniProt: Q05716
• Field of Research: Apoptosis; Tumor immunity.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 100 ng/mL
• Sample Type: Serum, plasma and other biological fluids..
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.56 ng/mL