IL12A ELISA Kit (Mouse)

The coefficient of variation of both intra-assay and inter-assay were less than 10%.

7732-18-5

Tumor necrosis factor

DIF, TNFA, TNFSF2, TNLG1F, TNF-alpha

7124

Human

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

ELISA Kit

ELISA

Colorimetric, OD450 nm

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture, supernates, serum, and plasma

7 pg/ml

Human

Components 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody Components Components 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials Streptavidin-HRP solution, 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody

TNF

TNF