IFNG ELISA Kit (Human)

• Reproducibility:

  The coefficient of variation of both intra-assay and inter-assay were less than 10%.

• Gene Name:

  Interferon, gamma

• Gene Aliases:

  IFG, IFI, IMD69

• Gene ID:

  3458

• Reactivity:

  Human

• Target:

  This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Detection Method:

  Colorimetric, OD450 nm

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture, supernates, serum, and plasma

• Sensitivity:

  7 pg/ml

• Homology:

  Human

• Components:

  Components 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody Components Components 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials Streptavidin-HRP solution 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody

• NCBI Gene Symbol:

  IFNG

• Gene Name URL:

  IFNG

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_000619