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EGF ELISA Kit (Human)

CAT: 0247-OKAA00001-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKAA00001-96WSize:96 Well
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Reproducibility
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
CAS Number
7732-18-5
Gene Name
Epidermal growth factor
Gene Aliases
URG, HOMG5
Gene ID
1951
Reactivity
Human
Target
This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Type
ELISA Kit
Applications
ELISA
Detection Method
Colorimetric, OD450 nm
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture, supernates, serum, and plasma
Sensitivity
16 pg/ml
Homology
Human
Components
Components 1 vial human EGF Standard lyophilized, 20 ng/ml upon reconstitution 1 vial concentrated Biotin-Conjugate anti-human EGF antibody 1 vial Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution Components Components 1 vial human EGF Standard lyophilized, 20 ng/ml upon reconstitution 1 vial human EGF Standard lyophilized, 20 ng/ml upon reconstitution 1 vial concentrated Biotin-Conjugate anti-human EGF antibody 1 vial concentrated Biotin-Conjugate anti-human EGF antibody 1 vial Streptavidin-HRP solution 1 vial Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution
NCBI Gene Symbol
EGF
Gene Name URL
EGF
Nucleotide Accession Number
NM_001178130.2

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