Lentivirus and Retrovirus Purification Kit

• Description:

  BioVision’s Lentivirus & Retrovirus Purification Kit is used to capture, concentrate and store lentiviruses and retroviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally, viruses are purified by ultracentrifugation using CsCl, which is time consuming and is limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Lentivirus (LV)-Retrovirus (RV)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.

• Reactivity:

  All

• Applications:

  PCR, western blot, ELISA, transfection directly with viruses bound to magnetic beads or eluted from the magnetic beads (free of beads).

• Detection Method:

  Lentiviruses and Retroviruses can be captured, concentrated and stored using magnetic beads

• Features & Benefits:

  Does not need ultracentrifugation, High yield and fast concentration (2 to 1000X), Ideal for cell culture transduction or infection, Purify viruses in 30-45 min

• Shipping Conditions:

  RT

• Storage Conditions:

  4°C

• Shelf Life:

  12 months