TIMP-2 (Human) ELISA Kit

• Description:

  TIMP metallopeptidase inhibitor 2 is also known as TIMP-2, which belongs to the protease inhibitor I35 (TIMP) family. This family proteins are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, TIMP2 has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. BioVision’s TIMP-2 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of TIMP-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human TIMP-2. The concentration of Human TIMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

• Reactivity:

  Human

• Applications:

  in-vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.

• Detection Method:

  Sandwich ELISA, Absorbance (450 nm)

• Features & Benefits:

  Specificity: No Significant cross-reactivity or interference between Human TIMP-2 and analogues was observed.,Precision: Coefficient of variation is < 10%,Sensitivity: 0.10 ng/mL,Detection Range: 0.16-10 ng/mL

• Shipping Conditions:

  Gel Pack

• Storage Conditions:

  4ºC

• Shelf Life:

  6 months

• CAS Number:

  7732-18-5