Products for Research Use Only
RegisterLogin

IL6 Antibody

CAT: 0013-GTR18187511Size: 1 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18187511Size:1 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
IL6 antibody
Product Name Alternative
HSF, Hybridoma growth factor, HGF, Hybridoma plasmacytoma growth factor, IL 6, IL6 protein, IFNB2, Interferon beta 2, BSF2, CDF, B-Cell Differentiation Factor, B-cell stimulatory factor 2, CTL differentiation factor, rabbit anti-Interleukin 6 Antibody, rabbit anti-IL-6
UniProt
P05231
Reactivity
Human
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Target
IL6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
80 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000
Purity
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts) . This product has minimal reactivity with mouse IL-6.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
IL-6 antibody has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, and immunoprecipitation. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC) . PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Tested Applications
DOT, ELISA, IHC, IP, WB
NCBI Accession Number
NP_000591.1
Host or Source
Rabbit
Preservative
None
Isotype
Antiserum

Related Products

CatalogName
GTR18225737IL6R/IL6 Receptor Antibody
GTR18221828IL6R/IL6 Receptor Antibody
GTR18221816IL6R/IL6 Receptor Antibody
GTR18224649IL6R/IL6 Receptor Antibody
GTR18579621IL6 Biosimilar Antibody
GTR18579042-01IL6 Biosimilar Antibody
GTR18796163-01IL6 Biosimilar Antibody
GTR18796154-01IL6 Biosimilar Antibody
GTR18578795-01IL6 Biosimilar Antibody
GTR18579381-01IL6 Biosimilar Antibody

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
DF8746-01ASAP1 antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
E36403ZNF98 Antibody Antibody
GWB-250026Lck antibody Antibody
E8M1007-4HSP60 Antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
GWB-5C6547MAD1L1 antibody [9B10] Antibody

Popular Products