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Gag-pro-pol Antibody

CAT: 0013-GTR18187425Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187425Size:25 µL
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Description
Gag-pro-pol antibody
Product Name Alternative
Rabbit anti-MMTV antibody, rabbit anti-mouse mammary tumor virus capsid protein antibody, Gag-Pro-Pol, Mouse mammary tumor virus (strain C3H), MMTV capsid, Reverse transcriptase/ribonuclease H, Capsid protein p27
UniProt
P11283
Reactivity
Virus
Immunogen
MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus capsid protein tagged with His.
Target
Gag-pro-pol
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Concentration
77mg/ml
Dilution
ELISA: 1:10,000-1:20,000, WB: 1:1000-1:2000
Purity
Mouse mammary tumor virus capsid antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01% and cross adsorbed with 6X HIS. A BLAST analysis was used to suggest cross-reactivity with MMTV from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 26.7 kDa in size corresponding to MMTV capsid protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

UniProtKB · P11283

Gag-Pro-Pol polyprotein

POL_MMTVC · Mouse mammary tumor virus (strain C3H)

View on UniProt ↗
Primary accession
P11283
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
gag-pro-pol
Protein existence
1: Evidence at protein level
Organism
Mouse mammary tumor virus (strain C3H) (MMTV)
Taxonomy ID
11759
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q9IZT3
Protein keywords

Molecular function

Aspartyl proteaseDNA-bindingDNA-directed DNA polymeraseEndonucleaseHydrolaseMultifunctional enzymeNucleaseNucleotidyltransferaseProteaseRNA-bindingRNA-directed DNA polymeraseTransferaseViral nucleoprotein

Cellular component

Capsid proteinViral matrix proteinVirion

Technical term

Direct protein sequencingReference proteome

Biological process

DNA integrationDNA recombinationViral genome integrationVirus entry into host cell

PTM

LipoproteinMyristatePhosphoprotein

Ligand

MagnesiumMetal-bindingNucleotide-bindingZinc

Domain

RepeatZinc-finger

Coding sequence diversity

Ribosomal frameshifting

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