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HIST1H3A Antibody

CAT: 0013-GTR18187392Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187392Size:25 µL
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Description
HIST1H3A antibody
Product Name Alternative
Rabbit anti-Histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody
UniProt
P68431
Reactivity
Human
Immunogen
Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
Target
HIST1H3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
83 mg/ml
Dilution
ELISA: 1:10000, IHC: 1:100, WB: 1:2000
Purity
Anti-Histone H3 is directed against the human histone3 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic (mouse, rat, chicken, dog, monkey, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly) . Cross-reactivity with histone-3 from other sources have not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Histone H3 antibody has been tested for use in ELISA, IHC, and western blot. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_003520.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
Antiserum

UniProtKB · P68431

Histone H3.1

H31_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P68431
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
H3C1, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3C10, H3C11, H3C12
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A0PJT7, A5PLR1, P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9, Q6NXU4, Q71DJ3, Q93081
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationCitrullinationHydroxylationIsopeptide bondLipoproteinMethylationPhosphoproteinUbl conjugation

Cellular component

ChromosomeNucleosome coreNucleus

Disease

Disease variant

Molecular function

DNA-binding

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