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Angpt1 Antibody

CAT: 0013-GTR18187340Size: 200 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187340Size:200 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Angpt1 antibody
Product Name Alternative
Rabbit anti-Angiopoietin 1 Antibody, rabbit anti-ANG1 antibody, AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody
UniProt
O08538
Reactivity
Human, Mouse
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Target
Angpt1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cardiovascular Research
Concentration
90 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, IHC: 1:200-1:800, WB: 1:500-1:2,000
Purity
Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
46048213
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

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