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Angpt2 Antibody

CAT: 0013-GTR18187339Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187339Size:25 µL
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Description
Angiopoietin 2 antibody
Product Name Alternative
Rabbit anti-Angiopoietin 2 Antibody, rabbit anti-ANG2 antibody, AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody
UniProt
O35608
Reactivity
Human, Mouse
Immunogen
Anti-Angiopoietin 2 Antiserum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Target
Angpt2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cardiovascular Research
Concentration
80 mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, IHC: 1:200-1:500, WB: 1:500 - 1:2,000
Purity
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Angiopoietin 2 Antibody is tested in WB, ICC, and IHC. Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.
Tested Applications
ELISA, IHC, Multiplex Assay, WB
NCBI Accession Number
NP_031452.2
Host or Source
Rabbit
Preservative
0.1% (w/v) Sodium Azide
Isotype
Antiserum

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