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NFYA Antibody

CAT: 0013-GTR18187289Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187289Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NFYA antibody
Product Name Alternative
Rabbit anti-NF-Y antibody, Nuclear transcription factor Y subunit alpha, Nuclear transcription factor Y subunit A, NF-YA, CAAT box DNA-binding protein subunit A
UniProt
P23511
Reactivity
Human
Immunogen
NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
NFYA
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
90 mg/ml
Dilution
ELISA: 1:20,000 - 1:100,000, Gel Supershift Assays: Use 2.0 μL, IHC: 1:400, WB: 1:2,000 - 1:10,000
Purity
Anti-NF-Y (A subunit) Antibody was prepared from monospecific antiserum by delipidation and defibrination.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-NF-Y (A subunit) Antibody was tested by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Negligible reactivity was observed against the B subunit of NF-Y. Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 μl per assay. Anti-NF-Y was tested in IHC using human prostate tissue with 10μg/mL. Specific conditions should be optimized by user.
Tested Applications
ELISA, Gel Supershift Assays, IHC, WB
NCBI Accession Number
CAI20286.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

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