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ALDOA Antibody

CAT: 0013-GTR18187270Size: 2 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187270Size:2 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ALDOA antibody
Product Name Alternative
Goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase
UniProt
P00883
Reactivity
Human, Rabbit
Immunogen
Aldolase [Rabbit Muscle]
Target
ALDOA
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
90 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Purity
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Aldolase has been tested by immunoprecipitation and western blot and is suitable to be assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_001075707.1
Host or Source
Goat
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum

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