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POLR2A Antibody

CAT: 0013-GTR18187211Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187211Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
POLR2A antibody
Product Name Alternative
RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A
UniProt
P24928
Reactivity
Human
Immunogen
Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
POLR2A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/ml
Dilution
ELISA: 1:10,000 - 1:50,000, WB: 1:1000
Purity
This affinity purified antibody is directed against human POL II. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and Chinese hamster.
Form
Liquid (sterile filtered)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG.
Tested Applications
ChIP, ELISA, WB
NCBI Accession Number
NP_000928.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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