Cas9 Antibody (HRP)

CAT: 0013-GTR18187163Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18187163Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Cas 9 antibody (Peroxidase)

Product Name Alternative

Rabbit anti-Cas9 antibody peroxidase conjugation, HRP conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

UniProt

Q99ZW2

Reactivity

Bacteria

Immunogen

Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Target

Cas9

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Molecular Biology

Concentration

1.0mg/mL

Dilution

ELISA: 1:25,000-1:30,000, IF: 1μg/mL, WB: 1μg/mL

Purity

Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Cas9 Peroxidase Conjugated Antibody has been tested for use in ELISA and suitable in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_269215.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · Q99ZW2

CRISPR-associated endonuclease Cas9/Csn1

CAS9_STRP1 · Streptococcus pyogenes serotype M1

View on UniProt ↗

Primary accession: Q99ZW2
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: cas9
Protein existence: 1: Evidence at protein level
Organism: Streptococcus pyogenes serotype M1
Taxonomy ID: 301447
Alternative names: —
EC number: 3.1.-.-
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureReference proteome

Biological process

Antiviral defense

Molecular function

DNA-bindingEndonucleaseExonucleaseHydrolaseNucleaseRNA-binding

Ligand

MagnesiumManganeseMetal-binding

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