Products for Research Use Only

MAPK1 Antibody (FITC)

CAT: 0013-GTR18187152Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187152Size:100 µg
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Description
ERK1/2 antibody (FITC)
Product Name Alternative
Rabbit anti-ERK2 antibody Fluorescein conjugation, FITC conjugated rabbit anti-ERK2 antibody, MAPK1, ERK 2, ERK-2, P42MAPK, PRKM1, PRKM2, MAPK 2, MAP kinase 2, Mitogen-activated protein kinase 2, p42-MAPK, MAP kinase isoform p42, Extracellular signal-regulated kinase 2, ERT1, MAP kinase 1, Mitogen-activated protein kinase 1
UniProt
P28482
Reactivity
Human, Mouse, Rat
Immunogen
Anti-ERK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Target
MAPK1
Clonality
Polyclonal
Conjugation
FITC
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:40,000, WB: 1μg/mL
Purity
This fluorescein conjugated affinity purified antibody is directed against human ERK2 protein. Anti-ERK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-ERK 2 (RABBIT) antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P28482

Mitogen-activated protein kinase 1

MK01_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28482
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MAPK1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MAP kinase 1, MAPK 1
EC number
2.7.11.24
Processing
—
Secondary accessions
A8CZ64
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCell cycleHost-virus interactionTranscriptionTranscription regulation

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell junctionCytoplasmCytoskeletonMembraneNucleus

Disease

Disease variant

Molecular function

DNA-bindingKinaseRepressorSerine/threonine-protein kinaseTransferase