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HIST1H3A Antibody

CAT: 0013-GTR18187138Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18187138Size:50 µg
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Description
Histone H3 K79-Me1 antibody
Product Name Alternative
Rabbit anti-Histone H3 monomethyl Lys79 antibody, H3K79me1, Anti-H3 K79-Me1, Histone H3 antibodies
UniProt
P84243
Reactivity
Human, Monkey, Mouse
Immunogen
Histone H3 K79-Me1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the Lys79 site of human Histone H3.
Target
HIST1H3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
0.50 mg/mL
Dilution
ChIP: 2 μg, IF: 1:50 - 1:100, WB: 0.5μg/mL
Purity
Anti-Histone H3 [Monomethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Histone H3 K79-Me1 antibody is tested in Western Blot, ChIP, Immunofluorescence, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
Tested Applications
ChIP, DOT, IF, Multiplex Assay, WB
NCBI Accession Number
NP_002098.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P84243

Histone H3.3

H33_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P84243
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
H3-3A, H3-3B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
P06351, P33155, Q5VV55, Q5VV56, Q66I33, Q9V3W4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationCitrullinationHydroxylationIsopeptide bondLipoproteinMethylationPhosphoproteinUbl conjugation

Cellular component

ChromosomeNucleosome coreNucleus

Disease

Disease variantIntellectual disability

Molecular function

DNA-binding

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