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PIK3CB Antibody

CAT: 0013-GTR18187044Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187044Size:25 µL
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Description
PIK3CB antibody
Product Name Alternative
Rabbit anti-PIK3CB antibody, rabbit anti-PI3K beta antibody, PKC3B. PKC3 beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-ß, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, PIK3C1
UniProt
P42338
Reactivity
Human
Immunogen
This affinity purified PIK3C-ß antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminal region of human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform.
Target
PIK3CB
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.09 mg/mL
Dilution
ELISA: 1:50,000, WB: 1:1000
Purity
Anti-PIK3C beta was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PIK3C beta protein. A BLAST analysis was used to suggest cross-reactivity with PI3K-beta based on a 100% homology from human sources with the immunizing sequence. Expect partial cross reactivity to PIK3C beta from mouse and rat (93% homology) sources. Cross-reactivity with PIK3C beta from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-PIK3C beta Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 122.7 kDa in size corresponding to PI3K beta by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_006210.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P42338

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform

PK3CB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P42338
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PIK3CB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta
EC number
2.7.1.153
Processing
—
Secondary accessions
D3DNF0, Q24JU2
Protein keywords

Ligand

ATP-bindingNucleotide-binding

Biological process

AutophagyCell adhesionEndocytosisLipid metabolism

Cellular component

CytoplasmNucleus

Molecular function

KinaseTransferase

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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